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ORIGINAL ARTICLE
Year : 2015  |  Volume : 35  |  Issue : 2  |  Page : 57-61

The mechanism of high transfection efficiency of human serum albumin conjugated polyethylenimine in A549 cells


1 School of Pharmacy, National Defense Medical Center, Neihu District, Taipei, Taiwan, Republic of China
2 Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan, Republic of China
3 School of Pharmacy, National Defense Medical Center, Neihu District, Taipei, Taiwan; Institute of Preventive Medicine, National Defense Medical Center, New Taipei, Taiwan, Republic of China

Correspondence Address:
Jiin-Long Chen
School of Pharmacy, National Defense Medical Center, No. 161, Section 6, Minquan East Road, Neihu, Taipei 114, Taiwan; Institute of Preventive Medicine, National Defense Medical Center, No. 192 Dapu Road, Sanxia District, New Taipei, 237, Taiwan
Republic of China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1011-4564.156009

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Background: In our previous study, HSA-PEI demonstrated high pDNA transfection efficiency and low cytotoxicity. Materials and Methods: In this study, the relationship between albumin receptors and the high pDNA transfection efficiency of HSA-PEI was investigated by fluorescent microscopy and flow-cytometer in A549 cells. Results: According to our results, the presence of an albumin receptor on A549 cells was confirmed via the application of a fluorescent tracer, FITC-HSA, and the dose-dependent competition of HSA. The transfection efficiency of HSA-PEI/pEGFP showed a dose-dependent inhibition when different amounts of HSA were added to the culture medium of the A549 cells. However, the inhibitory effect of HSA did not affect the transfection efficiency of some cationic transfection enhancement reagents, such as lipofectamine, jetPEI-RGD or PEI; their transfection was a result of contact between the positively charged reagents and the negatively charged cell surface. Conclusion: It was determined that, unlike other cationic reagents, the high transfection efficiency of HSA-PEI was not from the electronic effect but, instead, predominantly from its ligand effect.


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