Benefit of broccoli extract-sulforaphane prophylaxis in ventilator-induced lung injury
Chen-Liang Tsai1, Chih-Ying Changchien2, Chi-Huei Chiang3, Shan-Yueh Chang1, Ying-Chieh Chen1, Chih-Feng Chian1
1 Division of Pulmonary and Critical Care, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
2 Department of Internal Medicine, National Defense Medical Center, Tri-Service General Hospital, Taipei, Taiwan
3 Department of Chest, Taipei Veterans General Hospital, Taipei, Taiwan
Division of Pulmonary and Critical Care, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, 114 No. 325, Chengong Road, Sec. 2, Neihu District, Taipei City
Source of Support: None, Conflict of Interest: None
Background: Owig to the extensive use of mechanical ventilation, risks of acute lung injury are significant in the intensive care unit. Broccoli extract-sulforaphane (SFN) has been investigated as bioactive polyphenol in chronic lung diseases. Aim: The present study aimed to evaluate the preventive effect of SFN in a rat model of ventilator-induced lung injury. Methods: SFN supplement was administrated 30 min before intubation with the dosage of 3 mg/kg. Then, rats were assigned to receive ventilation with a high tidal volume of 40 mL/kg for 6 h, and low ventilation of 6 mL/kg served as controls. Results: The severity of pulmonary edema was mitigated in the SFN-pretreated group with decreased weight ratios of wet to dry lung and total lung to the body, respectively. From bronchoalveolar lavage, SFN treatment suppressed both leukocytes counts and cytokines production. Following ventilator-exerted oxidative burst with the rescue of glutathione level was identified in SFN-pretreated group. Besides, SFN-reduced cell apoptosis was confirmed by terminal deoxynucleotidyl transferase dUTP nick end labeling assay and cleavage of caspase-3. Western blotting from lung tissues revealed the upregulation of hemeoxygenase-1 with decreased nuclear factor κB and p38 phosphorylation in SFN-treated group. Conclusion: Our results elucidated the prophylaxis of broccoli extract-SFN could attenuate ventilator-induced oxidative stress, inflammation reaction, and pulmonary edema.